I Nyoman P. Aryantha1,2, Siti Khodijah Chaerun1*, Rizkita Rachmi Esyanti1, Nurmi D.P. Pangesti2, Dewi Jumiarni1 and Koki Toyota3
1School of Life Sciences and Technology, Bandung Institute of Technology, Ganesa 10, Bandung 40132, West Java, Indonesia
2Centre for Life Sciences, Bandung Institute of Technology, Ganesa 10, Bandung 40132, West Java, Indonesia
3Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
*Corresponding author: S.K. Chaerun (email: skchaerun@sith.itb.ac.id; skchaerun@gmail.com)
Abstract
The microbial diversity and activity of sediments from Saguling, Cirata and Jatiluhur Dam Reservoirs were studied to provide a better understanding of their biogeochemical and agricultural significance to enable them as natural fertilizers and artificial soils. Microbial respiration analysis revealed that CO2 evolved during 5 days of incubation time. Microbial biomass C of Saguling was higher than that of Jatiluhur and Cirata. BIOLOG analysis showed that sediment of Saguling showed the highest AWCD (sigmoid Average Well Color Development) which represented the microbial metabolic abilities. DGGE analysis of amplified bacterial 16S rDNA genes revealed some common bands in sediment from the three dam reservoirs, suggesting the presence of the same species of bacteria. Measurement of cellulose activity showed that sediment from Saguling had the highest activity of cellulose. The highest dehydrogenase activity was observed in Cirata, indicating that sediments in Cirata had the greatest activity of the active microbial population. Enumeration of aerobic microorganisms based on the hydrolysis of fluorescein diacetate [3′,6′-diacetylfluorescein (FDA)] showed that sediments from three dam reservoirs contained abundant microorganisms. The highest number of aerobic bacteria was found in Saguling, whereas that of anaerobic bacteria was observed in Cirata. From a microbiological point of view, our present data suggest that sediments in the three largest dam reservoirs (Saguling, Cirata, Jatiluhur) have high composition and enzymatic activity of microorganisms, thus playing a key role in some processes such as organic matter transformation and nutrient cycling which are advantageous for plant and further establishing the biogeochemical and agricultural significance of sediment.
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2nd USM Penang Inernationl Post Graduate Convention, 18-20 June 2008
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