Wednesday, October 15, 2008

OCCURRENCE OF TRITERPENOIDS AND POLYSACCHARIDES ON GANODERMA TROPICUM WITH GANODERMA LUCIDUM AS A REFERENCE

J. Australasian Mycologist, vol 20 (2) : 123-129 (2001)

I Nyoman P. Aryantha, Andi Adinda and Suswini Kusmaningati1)
Dept. of Biology ITB, Jalan Ganesha 10 Bandung 40132, Indonesia
1) IURC Life Sciences ITB, Jalan Ganesha 10 Bandung 40132, Indonesia

ABSTRACT

Local strain of Ganoderma tropicum has been analyzed for the appearance of triterpenoids and polysaccharides with a commercial Ganoderma lucidum as a reference. G. tropicum was isolated from Delonix regia tree, ornamental tree locally known as Flamboyant, on December 1999. The fungus is parasitic to the tree which is quite aggressive killing the tree within 1-5 years. The fruiting body of both G. tropicum and G. lucidum were obtained by growing them in sawdust after 3 month incubation. Analysis for triterpenoids was conducted by using silica gel-thin layer chromatography (TLC) with eluent of chloroform:methanol (10:1) and Carr-Price as well as Lieberman-Burchard as banding agents and observed under UV light (l366 nm). The same technique, except for the eluent n-buthanol:acetic acid:eter:water (9:6:3:1) and banding agent of aniline ftalate, was also used for detecting polysaccharides in form of their monomers (glucose, galactose, xylose and rhamnose) under normal light. Both triterpenes and polysaccharides were detected on G. tropicum and G. lucidum, indicating that local strain of G. tropicum also possesses medicinal value.
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Keywords : Ganoderma tropicum, Ganoderma lucidum, triterpenes, polysaccharides, medicinal mushroom

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