Jurnal Mikrobiologi Indonesia, Vol. 9 No. 2, hal 43-46
The Potency of IAA Producing Bacteria Isolates on Promotion The Growth of Mungbean Sprout [Vigna radiata (L.) Wilczek] in Hydroponic Condition
I Nyoman P. Aryantha[1], Dian P. Lestari., Nurmi Puri Dwi P.
Kelompok Penelitian dan Pengembangan (KPP) Ilmu Hayati LPPM-ITB
Gedung Litbang ITB (Lt.VI) Jl. Ganesha 10 Bandung 40132
ABSTRACT
Indole-3-acetic acid (IAA) is a key hormone for various aspects of plant growth and development. Liquid and powder products of five IAA producing bacteria (D2, D3 from Bacilli group and KB, LE, LC from actinomycetes group) were investigated in semi in vivo assay towards mungbean (Vigna radiata) growth. Liquid fermentation product without cell separation was diluted 20, 40 and 60 times in sterilized water and dried powder was suspended in four concentrations i.e 0.01, 0.1, 1 and 3 g per 100 ml of water. Biological assay was conducted towards 3-day-old mungbean seedlings with hydroponic method in 20 ml tubes at room temperature and light intensity of 40 lux. The length of seedlings and root branching were assessed over 4 days. The data were analyzed by ANOVA. The powder product of KB at concentration rate of 0.01 g/100 ml (IAA =0.021 μg/ml) gave the highest result (28.1 cm) in increasing seedling length (significant level : P < 0.05) compared with other treatments and control. For root branching, the liquid product of LC with 20 times dilution (IAA=1.82 μg/ml) gave the highest number (24.25) of branches (significant level : P < 0.05) compared with other treatments and control. These results indicate that these IAA-producing bacteria are potential to be used for promoting the growth of mungbean plant.
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[Keywords : Indole-3-acetic acid (IAA), Vigna radiata, Bacillus, Actinomycetes, Soil bacteria, Microbial Phytohormone] [1] Korespondensi dialamatkan ke : nyoman@bi.itb.ac.id, Tel/Fax : 022-2509165 HP. 0811229819
