International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java.
Nengah Dwianita Kuswytasari (1), I Nyoman Pugeg Aryantha (1,2)
1) RG Microbiology, Genetics and Molecular Biology School of Life Sciences and Technology ITB
2) Center for Life Sciences ITB
ABSTRACT
The presence of bacterial endosymbiont on some species of aphids has been studied by using molecular approach. Aphids were collected from BALITSA vegetable field in Lembang West Java. Four methods for DNA isolation were applied, but only modified method of Fukatsu (1999) was successfully isolated the high molecular weight of aphid DNA along with endosymbiont DNA. Polimerase Chain Reaction (PCR) technique was employed to amplify the DNA by using 16SA1 and SB1 primers. The primers (5’-AGAGTTTGATCMTGGCTCAG-‘3[forward], 5’-TACGGYTACCTTGTTACGACTT-‘3[reverse]) were designed to amplify general eubacterial 16S rDNA with a total length 1500 bp of amplification result. Amplification results show that only Aphis gossypii (Glover) give a positive result of bacterial endosymbiont. Other species of aphid [i.e Aphis craccivora (Koch), Myzus ornatus (Laing), Myzus persicae (Sulzer), Lipaphis erysimii (Kaltenbach), and Rhopalosiphum rufiabdominalis (Sasaki)] did not give any positive result of eubacterial endosymbiont. Perhaps there is no endosymbiont exist on these species or they have other kind of endosymbiont rather than eubacterial endosymbiont. It is also found that bacterial endosymbiont DNA can not be well preserved with acetone 100% over 1, 3, 6 and 9 months.
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Keywords : Bacterial endosymbiont, aphid, 16S rDNA
Monday, October 20, 2008
BIODEGRADATION OF CYANIDE BY BACTERIAL ISOLATE FROM TAPIOCA WASTE ORIGIN FROM TASIKMALAYA-WEST JAVA
International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java.
Yadi Haryadi (1) and I Nyoman Pugeg Aryantha (1,2)
1) Center for Life Sciences ITB
2) RG Microbiology, Genetics and Molecular Biology School of Life Sciences and Technology
ABSTRACT
Research on the potency of bacterial isolate to degrade cyanide has been conducted. The isolate was taken from contaminant soil at tapioca waste water treatment in Cikatomas, Tasikmalaya-West Java. The study was begin by isolation of bacterial that can degrade cyanide using liquid basal medium containing cyanide 50 ppm + 1 % (w/v) glucose (Akcil & Mudder, 2003). The isolate was then purified by using spread method on solid basal medium. One isolate identified as Paracoccus sp. was found to potential as biodegradation agent for cyanide. The isolate was adapted in liquid basal medium containing 200 ppm cyanide + 1 % (b/v) glucose. This activated inoculums was then used for further treatments. The next step of trial was the biodegradation of cyanide up to 300 ppm. After 60 hours incubation, approximately 68,2% of cyanide was degraded. The cyanide was degraded at optimum rate of 8,096 ppm hour-1. The last concentration of cyanide was 16,684 ppm. Ammonia was produced during the process in amount of 0,0286 - 83,7667 ppm. Ammonia was generated from cyanide and the significant amount occurred after most of cyanide disappeared. The conclusion of this research is that Paracoccus sp. which was isolated from tapioca waste can degrade up to 68,20 % of 300 ppm cyanide solution during 60 hours incubation with formation of ammonia.
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Keywords : Cyanide biodegradation, Paracoccus sp., tapioca waste, bioremediation
Yadi Haryadi (1) and I Nyoman Pugeg Aryantha (1,2)
1) Center for Life Sciences ITB
2) RG Microbiology, Genetics and Molecular Biology School of Life Sciences and Technology
ABSTRACT
Research on the potency of bacterial isolate to degrade cyanide has been conducted. The isolate was taken from contaminant soil at tapioca waste water treatment in Cikatomas, Tasikmalaya-West Java. The study was begin by isolation of bacterial that can degrade cyanide using liquid basal medium containing cyanide 50 ppm + 1 % (w/v) glucose (Akcil & Mudder, 2003). The isolate was then purified by using spread method on solid basal medium. One isolate identified as Paracoccus sp. was found to potential as biodegradation agent for cyanide. The isolate was adapted in liquid basal medium containing 200 ppm cyanide + 1 % (b/v) glucose. This activated inoculums was then used for further treatments. The next step of trial was the biodegradation of cyanide up to 300 ppm. After 60 hours incubation, approximately 68,2% of cyanide was degraded. The cyanide was degraded at optimum rate of 8,096 ppm hour-1. The last concentration of cyanide was 16,684 ppm. Ammonia was produced during the process in amount of 0,0286 - 83,7667 ppm. Ammonia was generated from cyanide and the significant amount occurred after most of cyanide disappeared. The conclusion of this research is that Paracoccus sp. which was isolated from tapioca waste can degrade up to 68,20 % of 300 ppm cyanide solution during 60 hours incubation with formation of ammonia.
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Keywords : Cyanide biodegradation, Paracoccus sp., tapioca waste, bioremediation
Effect of oyster mushroom mycelial water extract on total blood cholesterol level of
International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java. P. 160.
Afifah BS.1,2, INP. Aryantha1, Suswini K.1, Yuli S.21. Center for Life Science ITB, Jalan Ganesha 10 Bandung 401322. Department of Pharmacy UNJANI, Jl. Terusan Jenderal Sudirman Cimahi
ABSTRACT
A study on the effect of oyster mushroom (Pleurotus ostreatus) mycelial water extract on total blood cholesterol level has been conducted using Wistar female rats with high cholesterol diet and prophylthiouracil (PTU) as hypercholesterolemic inducing agent. The animal were divided into two dose-level groups and one control group, each group consists of six rats. In the cholesterol inhibition method, both high cholesterol diet and PTU were administered simultaneously with the mycelial extract orally. In the cholesterol reducing method, high cholesterol diet and PTU were given over 4 weeks and then followed by anticholesterol treatment with the mycelial extract. Two doses level of the mycelial extract, 55 and 110 mg/kg body weight were applied respectively. Total blood cholesterol level was observed once a week over 4 weeks. As hypercholesterolemic inhibitor, the both doses level relatively could inhibit about 9.34% and 9.61% respectively the increasing of cholesterol level significantly (P=0.05) at day-21 (3rd week) compared with control. As hypercholesterolemic reducer, 110 mg/kg body weight of the mycelial extract could reduce relatively about 59.73% hypercholesterolemia at day-7 (1st week) compared with control. It can be concluded that the mycelial water extract of oyster mushroom was able to inhibit and to reduce relatively hypercholesterolemia in female rats.
-------------------------------------------------------------------------------------------Keywords : oyster mushroom, mycelial water extrac, hypercholesterolemia
Afifah BS.1,2, INP. Aryantha1, Suswini K.1, Yuli S.21. Center for Life Science ITB, Jalan Ganesha 10 Bandung 401322. Department of Pharmacy UNJANI, Jl. Terusan Jenderal Sudirman Cimahi
ABSTRACT
A study on the effect of oyster mushroom (Pleurotus ostreatus) mycelial water extract on total blood cholesterol level has been conducted using Wistar female rats with high cholesterol diet and prophylthiouracil (PTU) as hypercholesterolemic inducing agent. The animal were divided into two dose-level groups and one control group, each group consists of six rats. In the cholesterol inhibition method, both high cholesterol diet and PTU were administered simultaneously with the mycelial extract orally. In the cholesterol reducing method, high cholesterol diet and PTU were given over 4 weeks and then followed by anticholesterol treatment with the mycelial extract. Two doses level of the mycelial extract, 55 and 110 mg/kg body weight were applied respectively. Total blood cholesterol level was observed once a week over 4 weeks. As hypercholesterolemic inhibitor, the both doses level relatively could inhibit about 9.34% and 9.61% respectively the increasing of cholesterol level significantly (P=0.05) at day-21 (3rd week) compared with control. As hypercholesterolemic reducer, 110 mg/kg body weight of the mycelial extract could reduce relatively about 59.73% hypercholesterolemia at day-7 (1st week) compared with control. It can be concluded that the mycelial water extract of oyster mushroom was able to inhibit and to reduce relatively hypercholesterolemia in female rats.
-------------------------------------------------------------------------------------------Keywords : oyster mushroom, mycelial water extrac, hypercholesterolemia
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