Suppression on the Aflatoxin-B production and the growth of Aspergillus flavus by Lactic Acid Bacteria

Biotechnology 6 (2) 2007 : 257-262

INyoman Pugeg Aryantha[1]
Arina Tri Lunggani

Pusat Ilmu Hayati dan Departemen Biologi FMIPA ITB
Jalan Ganesha 10 Bandung 40132
Email : nyoman@bi.itb.ac.id

ABSTRACT
Aflatoxins are highly toxic secondary metabolites produced during the growth of several fungi, especially Aspergillus fiavus. Aflatoxin-B (AFB) is one of them which may contaminate a wide variety organic product including human food and animal feed. The toxin causes serious health problem when consumed by human or animals. This research was aimed to study the potency of Lactic Acid Bacteria (LAB) in the inhibition of A. flavus growth and the production of Aflatoxin-B. Three species of LAB i.e. Laciobacillus delbrueckii, L. fermentum and L. plantarum were investigated for their abality in supressing and degrading of Aflatoxin-B as well as inhibiting the fungal growth. The trial was designed in three variations by challenging the fungi with each LAB species, before, at the same time and after fungal inoculation. It was found that all species of LAB are potential to inhibit fungal growth. Furthermore, Aflatoxin-B was also able to be reduced in the medium by all species. L. fermentum gave the strongest degradation of Aflatoxin-B, followed by L. delbrueckii and L. plantarum during 15 days incubation with a reduction rate of 0.1953 mg/L, 0.30 8 mg/L and 0.4463 mg/L respectively. In conclusion, this study was able to demonstrate that lactic acid bacteria are able to suppress the growth of A. flavus and also able to ddegrade the Aflatoxin-B.
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Keyword; Lactic acid bacteria, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus plantarum, Aspergillus flavus, Aflatoxin-B
1 Korespondensi di alamatkan ke : I Nyoman P. Aryantha