International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java.
Yadi Haryadi (1) and I Nyoman Pugeg Aryantha (1,2)
1) Center for Life Sciences ITB
2) RG Microbiology, Genetics and Molecular Biology School of Life Sciences and Technology
ABSTRACT
Research on the potency of bacterial isolate to degrade cyanide has been conducted. The isolate was taken from contaminant soil at tapioca waste water treatment in Cikatomas, Tasikmalaya-West Java. The study was begin by isolation of bacterial that can degrade cyanide using liquid basal medium containing cyanide 50 ppm + 1 % (w/v) glucose (Akcil & Mudder, 2003). The isolate was then purified by using spread method on solid basal medium. One isolate identified as Paracoccus sp. was found to potential as biodegradation agent for cyanide. The isolate was adapted in liquid basal medium containing 200 ppm cyanide + 1 % (b/v) glucose. This activated inoculums was then used for further treatments. The next step of trial was the biodegradation of cyanide up to 300 ppm. After 60 hours incubation, approximately 68,2% of cyanide was degraded. The cyanide was degraded at optimum rate of 8,096 ppm hour-1. The last concentration of cyanide was 16,684 ppm. Ammonia was produced during the process in amount of 0,0286 - 83,7667 ppm. Ammonia was generated from cyanide and the significant amount occurred after most of cyanide disappeared. The conclusion of this research is that Paracoccus sp. which was isolated from tapioca waste can degrade up to 68,20 % of 300 ppm cyanide solution during 60 hours incubation with formation of ammonia.
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Keywords : Cyanide biodegradation, Paracoccus sp., tapioca waste, bioremediation
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