Shinta Asharina1 and I Nyoman P. Aryantha1,2
1Microbiology-Genetics-Cell Molecular Biology Division, School of Life Sciences and Technology
2Center for Life Sciences
Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132
Email : nyoman@sith.itb.ac.idABSTRACT
Microbial Fuel Cell (MFC) is a new energy generating system using microbes ability to produce electricity directly from renewable materials. Pseudomonas sp, an iron bacterium has been known for its ability to convert chemical energy from mineral intto electrical voltage through MFC system. In this study, the performance of Pseudomonas sp was evaluated after being treated with several doses of Gamma ray. Gamma ray, which is a strong ionizing agent can result in the damage of DNA. Radiation doses from 0.1-1 KGy were exposed to bacterial cell suspension before regrown on agar media. The result showed that, the survival rate of bacteria was the lowest at the dose of 1 KGy and slightly higher at the dose of 0.8 KGy treatment. The survived isolates from each dose were then chosen randomly before electrical voltage evaluation in MFC system. Iron modified medium consisting of Sidoarjo mud, Urea, and FeCl3 was used for growing the mutant candidates. The chosen mutant isolate (dose 0.8 KGy) produced 1.353 V. The electrical voltage measurement of the chosen mutant and wild type were compared using MFC system during 5 days. The initial pH of modified medium was adjusted at 7. The result showed that there was no significant differences on electrical voltage production between the mutant and wild type isolate. This suggests that random mutation through Gamma irradiation on this iron bacterium (Pseudomonas sp.) seems to be instable. Further study needs to be conducted to find out the optimum radiation dose for this iron bacterium in order to obtain a higher electrical voltage.
------------------------------------------------------
International Conference And Exhibition-Science & Technology In Biomass Production: Optimizing University-Industry Collaboration, ITB Bandung, 25-26 November 2009
No comments:
Post a Comment