The presence of bacterial endosymbiont in some species of aphid from Lembang, West Java

International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java.

Nengah Dwianita Kuswytasari (1), I Nyoman Pugeg Aryantha (1,2)

1) RG Microbiology, Genetics and Molecular Biology School of Life Sciences and Technology ITB
2) Center for Life Sciences ITB

ABSTRACT

The presence of bacterial endosymbiont on some species of aphids has been studied by using molecular approach. Aphids were collected from BALITSA vegetable field in Lembang West Java. Four methods for DNA isolation were applied, but only modified method of Fukatsu (1999) was successfully isolated the high molecular weight of aphid DNA along with endosymbiont DNA. Polimerase Chain Reaction (PCR) technique was employed to amplify the DNA by using 16SA1 and SB1 primers. The primers (5’-AGAGTTTGATCMTGGCTCAG-‘3[forward], 5’-TACGGYTACCTTGTTACGACTT-‘3[reverse]) were designed to amplify general eubacterial 16S rDNA with a total length 1500 bp of amplification result. Amplification results show that only Aphis gossypii (Glover) give a positive result of bacterial endosymbiont. Other species of aphid [i.e Aphis craccivora (Koch), Myzus ornatus (Laing), Myzus persicae (Sulzer), Lipaphis erysimii (Kaltenbach), and Rhopalosiphum rufiabdominalis (Sasaki)] did not give any positive result of eubacterial endosymbiont. Perhaps there is no endosymbiont exist on these species or they have other kind of endosymbiont rather than eubacterial endosymbiont. It is also found that bacterial endosymbiont DNA can not be well preserved with acetone 100% over 1, 3, 6 and 9 months.
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Keywords : Bacterial endosymbiont, aphid, 16S rDNA

BIODEGRADATION OF CYANIDE BY BACTERIAL ISOLATE FROM TAPIOCA WASTE ORIGIN FROM TASIKMALAYA-WEST JAVA

International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java.

Yadi Haryadi (1) and I Nyoman Pugeg Aryantha (1,2)
1) Center for Life Sciences ITB
2) RG Microbiology, Genetics and Molecular Biology School of Life Sciences and Technology

ABSTRACT

Research on the potency of bacterial isolate to degrade cyanide has been conducted. The isolate was taken from contaminant soil at tapioca waste water treatment in Cikatomas, Tasikmalaya-West Java. The study was begin by isolation of bacterial that can degrade cyanide using liquid basal medium containing cyanide 50 ppm + 1 % (w/v) glucose (Akcil & Mudder, 2003). The isolate was then purified by using spread method on solid basal medium. One isolate identified as Paracoccus sp. was found to potential as biodegradation agent for cyanide. The isolate was adapted in liquid basal medium containing 200 ppm cyanide + 1 % (b/v) glucose. This activated inoculums was then used for further treatments. The next step of trial was the biodegradation of cyanide up to 300 ppm. After 60 hours incubation, approximately 68,2% of cyanide was degraded. The cyanide was degraded at optimum rate of 8,096 ppm hour-1. The last concentration of cyanide was 16,684 ppm. Ammonia was produced during the process in amount of 0,0286 - 83,7667 ppm. Ammonia was generated from cyanide and the significant amount occurred after most of cyanide disappeared. The conclusion of this research is that Paracoccus sp. which was isolated from tapioca waste can degrade up to 68,20 % of 300 ppm cyanide solution during 60 hours incubation with formation of ammonia.
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Keywords : Cyanide biodegradation, Paracoccus sp., tapioca waste, bioremediation

Effect of oyster mushroom mycelial water extract on total blood cholesterol level of

International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java. P. 160.

Afifah BS.1,2, INP. Aryantha1, Suswini K.1, Yuli S.21. Center for Life Science ITB, Jalan Ganesha 10 Bandung 401322. Department of Pharmacy UNJANI, Jl. Terusan Jenderal Sudirman Cimahi

ABSTRACT

A study on the effect of oyster mushroom (Pleurotus ostreatus) mycelial water extract on total blood cholesterol level has been conducted using Wistar female rats with high cholesterol diet and prophylthiouracil (PTU) as hypercholesterolemic inducing agent. The animal were divided into two dose-level groups and one control group, each group consists of six rats. In the cholesterol inhibition method, both high cholesterol diet and PTU were administered simultaneously with the mycelial extract orally. In the cholesterol reducing method, high cholesterol diet and PTU were given over 4 weeks and then followed by anticholesterol treatment with the mycelial extract. Two doses level of the mycelial extract, 55 and 110 mg/kg body weight were applied respectively. Total blood cholesterol level was observed once a week over 4 weeks. As hypercholesterolemic inhibitor, the both doses level relatively could inhibit about 9.34% and 9.61% respectively the increasing of cholesterol level significantly (P=0.05) at day-21 (3rd week) compared with control. As hypercholesterolemic reducer, 110 mg/kg body weight of the mycelial extract could reduce relatively about 59.73% hypercholesterolemia at day-7 (1st week) compared with control. It can be concluded that the mycelial water extract of oyster mushroom was able to inhibit and to reduce relatively hypercholesterolemia in female rats.
-------------------------------------------------------------------------------------------Keywords : oyster mushroom, mycelial water extrac, hypercholesterolemia

The optimization of nutrient factors in spore production of Paecilomyces fumosoroseus (Wize) Brown & Smith with submerged – surface fermentation syste

Proceeding ITB, Vol. 32, (3) : 85-91 (2000)

Dini Jamiah Rayati; I Nyoman P. Aryantha and Purwo Arbianto

Department of Biology, Faculty of Mathematics and Sciences ITB
Jalan Ganesha 10 Bandung Indonesia

Paecilomyces fumosoroseus has been reported to be potential as a biocontrol agent for insect pests. The mass spore production of the fungus still needs to be developed. This research was trying to find out the optimum condition for producing the mass spore production of the fungus. At submerged-fermentation stage (vegetative growth), the nutrition factors (i.e. carbon, nitrogen source and C/N ratio) were optimized, whereas at surface-fermentation stage (sporulation) nutrient concentration was optimized. The results show that three kinds of carbon source (i.e glucose, maltose, and sucrose) gave the same mycelial growth for P. fumosoroseus. The organic nitrogen sources (i.e glysine, glutamine, serine, alanine, asparagines and a mixture of the first four amino acids) gave a better significant growth for P. fumosoroseus (a=0,05) compared with the inorganic -nitrogen source (sodium nitrate and ammonium chloride). The optimum C/N ratio for the mycelial growth of the fungus was found at 14 value. Finally, the optimum nutrient condition for sporulation was found in the stationary growth phase medium by transferring the harvested mycelium into a flat container. At this optimum condition the fungus produced an average of 4.5 x 109 spre per gram mycelium after 190 hours. These results suggests that the mass spore production of P. fumosoroseus could be done by using two steps fermentation system by using organic substrate containing amino acids and sugars such as wheat or rice bran and mollases.
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Biological control, Entomopathogenic fungus, fumosoroseus, two-stage fermentation

OCCURRENCE OF TRITERPENOIDS AND POLYSACCHARIDES ON GANODERMA TROPICUM WITH GANODERMA LUCIDUM AS A REFERENCE

J. Australasian Mycologist, vol 20 (2) : 123-129 (2001)

I Nyoman P. Aryantha, Andi Adinda and Suswini Kusmaningati1)
Dept. of Biology ITB, Jalan Ganesha 10 Bandung 40132, Indonesia
1) IURC Life Sciences ITB, Jalan Ganesha 10 Bandung 40132, Indonesia

ABSTRACT

Local strain of Ganoderma tropicum has been analyzed for the appearance of triterpenoids and polysaccharides with a commercial Ganoderma lucidum as a reference. G. tropicum was isolated from Delonix regia tree, ornamental tree locally known as Flamboyant, on December 1999. The fungus is parasitic to the tree which is quite aggressive killing the tree within 1-5 years. The fruiting body of both G. tropicum and G. lucidum were obtained by growing them in sawdust after 3 month incubation. Analysis for triterpenoids was conducted by using silica gel-thin layer chromatography (TLC) with eluent of chloroform:methanol (10:1) and Carr-Price as well as Lieberman-Burchard as banding agents and observed under UV light (l366 nm). The same technique, except for the eluent n-buthanol:acetic acid:eter:water (9:6:3:1) and banding agent of aniline ftalate, was also used for detecting polysaccharides in form of their monomers (glucose, galactose, xylose and rhamnose) under normal light. Both triterpenes and polysaccharides were detected on G. tropicum and G. lucidum, indicating that local strain of G. tropicum also possesses medicinal value.
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Keywords : Ganoderma tropicum, Ganoderma lucidum, triterpenes, polysaccharides, medicinal mushroom

Suppression of Phytophthora cinnamomi in potting mixes amended with uncomposted and composted animal manures

Artikel lengkap

Potensi Isolat Bakteri Penghasil IAA dalam Meningkatkan Pertumbuhan Kecambah Kacang Hijau [Vigna radiata (L.) Wilczek Pada Kondisi Hidroponik

Jurnal Mikrobiologi Indonesia, Vol. 9 No. 2, hal 43-46

The Potency of IAA Producing Bacteria Isolates on Promotion The Growth of Mungbean Sprout [Vigna radiata (L.) Wilczek] in Hydroponic Condition

I Nyoman P. Aryantha[1], Dian P. Lestari., Nurmi Puri Dwi P.
Kelompok Penelitian dan Pengembangan (KPP) Ilmu Hayati LPPM-ITB
Gedung Litbang ITB (Lt.VI) Jl. Ganesha 10 Bandung 40132
ABSTRACT

Indole-3-acetic acid (IAA) is a key hormone for various aspects of plant growth and development. Liquid and powder products of five IAA producing bacteria (D2, D3 from Bacilli group and KB, LE, LC from actinomycetes group) were investigated in semi in vivo assay towards mungbean (Vigna radiata) growth. Liquid fermentation product without cell separation was diluted 20, 40 and 60 times in sterilized water and dried powder was suspended in four concentrations i.e 0.01, 0.1, 1 and 3 g per 100 ml of water. Biological assay was conducted towards 3-day-old mungbean seedlings with hydroponic method in 20 ml tubes at room temperature and light intensity of 40 lux. The length of seedlings and root branching were assessed over 4 days. The data were analyzed by ANOVA. The powder product of KB at concentration rate of 0.01 g/100 ml (IAA =0.021 μg/ml) gave the highest result (28.1 cm) in increasing seedling length (significant level : P < 0.05) compared with other treatments and control. For root branching, the liquid product of LC with 20 times dilution (IAA=1.82 μg/ml) gave the highest number (24.25) of branches (significant level : P < 0.05) compared with other treatments and control. These results indicate that these IAA-producing bacteria are potential to be used for promoting the growth of mungbean plant.
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[Keywords : Indole-3-acetic acid (IAA), Vigna radiata, Bacillus, Actinomycetes, Soil bacteria, Microbial Phytohormone] [1] Korespondensi dialamatkan ke : nyoman@bi.itb.ac.id, Tel/Fax : 022-2509165 HP. 0811229819

Phosphonate (PO3-) effectiveness against Phytophthora cinnamomi Rands on Thryptomene calycina, Banksia grandis and Banksia spinulosa

Plant Pathology Journal 3(1) 2004 : 19-25
I Nyoman Pugeg Aryantha1* and David I. Guest2
1) Dept. of Biology and Research Development Group on Life Sciences, Institut Teknologi Bandung (ITB), Jalan Ganesha 10, Bandung 40132, Indonesia
Tlp./Fax. +62-22-2509165, Email : nyoman@bi.itb.ac.id
*) Corresponding author should be addressed
2) School of Botany, The University of Melbourne, Victoria-Australia


ABSTRACT

Our study shows that Potassium phosphonate has been proven to slow down the growth rate of P. cinnamomi in in vitro. Phosphonate drench as low as one gram per litre was effective in protecting Thryptomene calycina, Banksia grandis, and B. spinulosa in pot and field trials. In glass house trials, concentrations as low as one gram per litre (drench) significantly suppressed the P. cinnamomi population. Concentrations over two and a half gram per litre were phytotoxic to all plant species tested. The most sensitive species was B. spinulosa. Phosphonate [5 gL-1] killed all B. spinulosa plants in seven weeks, therefore it must be used with a great care. Phosphonate treatment alone was effective protecting plants from disease in the field, but did not result in high plant health. Despite new root growth in pot trials after seven weeks, poor growth was commonly observed on T. calycina after 14 months in field trials. This suggests that phosphonate is not suitable as sole application particularly for the long term. A combination of phosphonate with compost as well as antagonist as an integrated management will be a good alternative for P. cinnamomi management in the future.
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Keywords : Phosphonate, Phytophthora cinnamomi, Thryptomene calycina, Banksia grandis, Banksia spinulosa, phytotoxic, Integrated control

Suppression of Phytophthora cinnamomi Rands by mycoprasitic and antagonistic microorganisms

Plant Pathology Journal : 5(3) 2006, 291-298
Suppression of Phytophthora cinnamomi Rands by mycoprasitic and antagonistic microorganisms

I Nyoman P. Aryantha [1] and David I. Guest2
1) Dept. of Biology and Research Development Group on Life Sciences, Institut Teknologi Bandung (ITB), Jalan Ganesha 10, Bandung 40132, Indonesia
Tlp./Fax. +62-22-2509165
Email : nyoman@bi.itb.ac.id
2) School of Botany, The University of Melbourne, Victoria Australia
Running title: Antagonists from composted manuresSuppression of Phytophthora cinnamomi Rands by mycoprasitic and antagonistic microorganisms

ABSTRACT

We have isolated bacteria and fungi from composted chicken, sheep, cow and horse manure and screened each isolate for its ability to suppress P. cinnamomi in dual-culture in vitro plate and slide assays. Of the 180 isolates, including 31 actinomycetes, 64 fungi, 44 fluorescent pseudomonads and 41 endospore-forming bacteria, 45 isolates significantly inhibited the growth of P. cinnamomi. The inhibitory microbes were identified based on morphological and biochemical characters including 24 fungi (including Trichoderma spp., Gliocladium penicillioides and Fusarium spp.), 10 actinomycetes (all Streptomyces sp.), 7 fluorescent pseudomonads (Pseudomonas sp.) and 4 endospore-forming bacteria (Bacillus sp.). The most common mode of action observed was antibiosis, although mycoparasitism, indicated by parallel hyphal growth, hyphal coiling, appressorium formation and direct penetration, was also observed with one isolate of Trichoderma. These results help to explain the role of microbes in the suppression and biological control of P. cinnamomi by composted manures.
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Keywords : Antagonism, Antibiosis, Mycoparasitism, Biocontrol, Phytophthora cinnamomi,
Bacillus, Pseudomonas, Actinomycete.
[1] Corresponding author should be addressed to

Suppression on the Aflatoxin-B production and the growth of Aspergillus flavus by Lactic Acid Bacteria

Biotechnology 6 (2) 2007 : 257-262

INyoman Pugeg Aryantha[1]
Arina Tri Lunggani

Pusat Ilmu Hayati dan Departemen Biologi FMIPA ITB
Jalan Ganesha 10 Bandung 40132
Email : nyoman@bi.itb.ac.id

ABSTRACT
Aflatoxins are highly toxic secondary metabolites produced during the growth of several fungi, especially Aspergillus fiavus. Aflatoxin-B (AFB) is one of them which may contaminate a wide variety organic product including human food and animal feed. The toxin causes serious health problem when consumed by human or animals. This research was aimed to study the potency of Lactic Acid Bacteria (LAB) in the inhibition of A. flavus growth and the production of Aflatoxin-B. Three species of LAB i.e. Laciobacillus delbrueckii, L. fermentum and L. plantarum were investigated for their abality in supressing and degrading of Aflatoxin-B as well as inhibiting the fungal growth. The trial was designed in three variations by challenging the fungi with each LAB species, before, at the same time and after fungal inoculation. It was found that all species of LAB are potential to inhibit fungal growth. Furthermore, Aflatoxin-B was also able to be reduced in the medium by all species. L. fermentum gave the strongest degradation of Aflatoxin-B, followed by L. delbrueckii and L. plantarum during 15 days incubation with a reduction rate of 0.1953 mg/L, 0.30 8 mg/L and 0.4463 mg/L respectively. In conclusion, this study was able to demonstrate that lactic acid bacteria are able to suppress the growth of A. flavus and also able to ddegrade the Aflatoxin-B.
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Keyword; Lactic acid bacteria, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus plantarum, Aspergillus flavus, Aflatoxin-B
1 Korespondensi di alamatkan ke : I Nyoman P. Aryantha

Efek pemberian ekstrak tubuh buah dan filtrat kultur cair Ganoderma tropicum terhadap penurunan kadar gula darah tikus putih galur Winstar

I.N.P. Aryantha1,2 dan S. Kusmaningati1

1Pusat Ilmu Hayati ITB
2Prodi Mikrobiologi Dep. Biologi ITB

ABSTRAK

Penelitian ini dilakukan untuk mengetahui pengaruh pemberian ekstrak air tubuh buah dan filtrat kultur cair cendawan Ganoderma lucidum (GL) dan Ganoderma tropicum (GT) terhadap penurunan kadar gula darah. Tubuh buah kedua cendawan ditumbuhkan dalam substrat serbuk kayu Albizia secara konvensional (metode bag log), sedangkan kultur cair miselium disiapkan dalam medium Potato Dextrose Broth dengan kondisi penumbuhan 7 hari inkubasi dinamis dan 21 hari statis dalam suhu ruang. Tubuh buah kering (kadar air sekitar 15%) diserbukkan dengan alat blender kering. Bubuk kering 10% dalam air (b/v) diekstrak dengan pemanasan pada suhu 90-95oC selama 48 jam, dilanjutkan dengan hidrolisis H2SO4 1M (100oC selama 8 jam), berikutnya dinetralkan dengan Ba(OH)2, lalu pemisahan endapan dengan pemusingan 5000 rpm, dan terakhir pengeringan dengan sistim hampa udara. Sementara itu, filtrat kultur cair dipisahkan dari biomasa miselium secara filtrasi lalu dikeringkan dengan oven suhu 50oC selama 7 hari sampai didapat kristal padat sebelum dijadikan dalam bentuk bubuk.

Tikus putih galur Winstar jantan sebelumnya dibuat hiperglikemi dengan diet glukosa 2500 mg/kg bb. selama 1 minggu.Pemberian ekstrak dilakukan secara oral yang dibagi dalam 6 kelompok perlakuan (5 tikus ulangan masing dosis perlakuan) sbb : (1) kontrol hanya diberi air matang; (2) pembanding obat (glibenklamid dosis 1 mg/kg bb); (3) filtrat kultur cair GL (dosis 500 dan 1000 mg/kg bb); (4) filtrat kultur cair GT (dosis 500 dan 1000 mg/kg bb); (5) ekstrak tubuh buah GL (dosis 175, 350 dan 700 mg/kg bb) dan (6) ekstrak tubuh buah GT (dosis 175, 350 dan 700 mg/kg bb).

Hasil uji ANOVA menunjukkan bahwa tidak ada perbedaan nyata antara semua kelompok, namun filtrat kultur cair GT dosis 1000 mg/kg bb terlihat mampu menurunkan (menahan peningkatan) kadar gula darah terbesar. Sedangkan ekstrak air tubuh buah GL rata-rata memberikan efek penurunan kadar gula darah terkecil. Pada 0,5-1 jam, rata-rata pemberian filtrat kultur cair memberi efek terhadap peningkatan kadar gula darah yang sebanding dengan kontrol dan terlihat kurang mampu jika dibandingkan dengan kelompok pembanding (obat). Setelah 1 jam, hanya filtrat kultur cair GT (1000 mg/kg bb) yang menurunkan kadar gula darah terbesar (dari semua dosis perlakuan termasuk pembanding), sedangkan filtrat kultur cair yang lain rata-rata hanya setara dengan kontrol dan tampak kurang mampu menurunkan kadar gula darah dibandingkan dengan pembanding. Perlakuan ekstrak tubuh buah rata-rata memberi pengaruh yang kurang lebih sama dengan kontrol, kecuali perlakuan ekstrak tubuh buah GT (700 mg/kg bb) yang tampak memberi pengaruh hampir sama dengan dengan pembanding.
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Kata Kunci : Medicinal mushroom, Ganoderma lucidum, Ganoderma tropicum, Lingchi, anti-diabetes, kadar gula darah, GTT (GlucoseTolerance Test)
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Kumpulan Abstrak Kongres IX Perhimpunan Mikrobiologi Indonesia, 25-26 Agustus
2005, Sanur, Bali, hal 30

My previous students

Undergraduate

1. Fajar Dwikusnoaji (1998-Elektro ITB)
2. Ira Fachira (1999)
3. Poppy Patricia (2000)
4. Dian Purnama Lestari (2000)
5. Ria Frenni (2000)
6. Dita Aryani (2001)
7. Andi Adinda Amata (2001)
8. Erika Nandina (2002)
9. Rubiyanti Dalimunthe (2002)
10. Rafika Agusriana Zal (2002-Farmasi ITB)
11. Telly Anggitawari (2003-Peternakan UNPAD)
12. Eva Ratih Anggraeni (2003-Peternakan Unpad)
13. Indah Sribanyu (2003-Peternakan Unpad)
14. Arnando Koswara (2003)
15. Reliita Veronika (2003)
16. Nia Kurnianingsih (2003)
17. Astrid Destyana (2004)
18. Grace (2004)
19. Brigitta Endah Wirastuti (2004)
20. Siskha Widayanti (2004)
21. Yuli Sumartini (2004-UNJANI)
22. Devi Ismidianty (2005)
23. Pretty Treciana (2005)
24. Arief Budiman (2006)
25. Amelia Anggaini (2006)
26. Mutiara Laksminingrum Sidharta (2007)
27. Rayhan Yusuf (2007)
28. Dian Karamita (2007)
29. Jamilah (2007)
30. Petrus Cahyadi (2008)
31. Dessy Dwiyanti (2008)
32. Myrna Haryanti (2008)
33. Hilda Nurlaeli (2008)
34. Rahmi Yusuf (2008)
35. Anggi Gravito (2009)
36. Mega Arfindari (2009)
37. Anggita Karlesa (2009)
38. Amalia Muntiyara P. (2009)
39. Mochamad Alqi H. (2009)
40. Diny Lucyanie (2009)
41. Shinta Asarina (2010)
42. Fauzia (2010)
43. Chandra Kurniawan (2010)
44. Rizka Dwi Nurhapsari (2010)
45. Raisa Canto (2010)
46. Muchammad Rizal Anshori (2010)
47. Siti Marfungah (2011)
48. Ahmad Effendi (2011)
49. Yulianto Wibowo (2011)
50. Irma (2011)
51. Laras Cempaka (2011)
52. Sophian Faturahman (2012)
53. Tamara Aprilia Sriyanti (2012)
54. Natalia Maria Theresia (2013)

Postgraduate (Master)

1. Kabelan Kunia (2000)
2. Siti Nursinar (2001)
3. Verania (2002)
4. Arina Tri Lunggani (2002)
5. Sri Redjeki (2002)
6. Arina Novilla (2005)
7. Betty Nurhayati (2005)
8. Tri Yuliana (2007)
9. Herti Endang Rosmayani (2007)
10. YIP Arry Miryanti (2007)
11. Dewi Jumiarni (2008)
12. Dewi Iriani (2008)
13. Rifky W Rachman (2008)
14. Yusuf Sofyan Efendi (2008)
15. Yati Supriatin (2009)
16. Rouland Ibnu Darda (2009)
17. Ida Munfarida (2009)
18. Reny Maria Angraeni (2009)
19. Anita Oktari (2010)
20. Ima Noor Puspitasari (2010)
21. Rafid Sanjaya (2011)
22. Yessi Annisa (2011)
23. Nurul Ihsani (2012)
24. Nurul Fitriani (2012)
25. Victor Mangila (2012)
26. Luthfia (2012)

Postgraduate (Doctorate)

1. Delianis Pringgenis (2003)
2. Sri Suhermiyati (UNPAD - 2003)
3. Enri Musnandar (UNPAD - 2004)
4. Umroh (2009)
5. Atira (UNPAD-2009)
6. Baiq Fatma (UPI-2010)
7. Baiq Viera (2012)

Publication and Award

JOURNAL

1.     Aryantha, I.P., R. Cross & D.I. Guest, 2000, Suppression of Phytophthora cinnamomi Rands in potting mixes amended with uncomposted and composted animal manure's, Phytopathology (J) 90 (7), 775-782.

2.     Rayati, J., I.P. Aryantha and P. Arbianto, 2000, The optimization of nutrition factors in spore production of Paecilomyces fumosoroseus (Wize) Brown & Smith with submerged-surface fermentation system, J. Proc. ITB, 32 (3) p 85-71

3.     Aryantha, I.P., A. Adinda & S. Kusmaningati, 2001, Occurrence of triperpenoids and polysaccharides on Ganoderma tropicum with Ganoderma lucidum as reference, J. Australasian Mycologist, v.20 (2) : 123-129

4.     Aryantha, I.P. & D. Guest, 2004, Phosphonate (PO3-) effectiveness against Phythophthora cinnamomi Rands on Tryptomene calycina, Banksia grandis and Banksia spinulosa, Plant Pathology Journal, Vol. 3 No. 1 p : 19-25

5.     Aryantha, IP., D.P. Lestari & N.P.D.Pangestu, 2004, Potensi isolat bakteri penghasil IAA dalam peningkatan pertumbuhan kecambah kacang hijau pada kondisi hidroponik, Jurnal Mikrobiologi Indonesia, Vol. 9 No. 2, hal 43-46

6.     Aryantha, IP & D.I. Guest, 2006, Mycoparasitic and antogistic inhibition on Phytophthora cinnamomi Rands by microbial agents isolated from manure compost, Plant Pathology Journal, 5(3) : 291-298.

7.     Syam'un E., A.Dahlan, INP. Aryantha, G.Suantika, 2006, Respons Dua Varietas Padi Terhadap Isolat Bakteri Azotobacter sp., Agrivigor. Vol 6, No 1 (2006)

8.     Aryantha, IP &  A. Trilunggani, 2007, Suppression on the Aflatoxin-B production and the growth of Aspergillus flavus by Lactic Acid Bacteria (Lactobacillus delbrueckii, Lactobacillus fermentum and Lactobacillus plantarum), Biotechnology Journal, Vol. 6(2), 257-262.

9.     Aryantha, INP., R. Arifudin, & Y. Mulyani, 2008, Penanda DNA mikrosatelit untuk karakterisasi kualitas bibit jamur kuping (Auricularia polytricha), JMS, 13 (1) : 7-15

10.   Umrah, T. Anggraeni, R.R. Esyanti, INP. Aryantha, 2009, Antagonisitas dan efektivitas Trichoderma sp. dalam menekan perkembangan Phytophthora palivora pada buah kakao, J. Agroland 16(1): 9-16

11.   Umrah, T. Anggraeni, R.R.Esyanti dan I.N.P. Aryantha, 2009, Pengembangan Formula Substrat Inokulum Trichoderma sp. Sebagai Agen Pengendali Hayati Terhadap Penyakit Busuk Buah Kakao, Jurnal Ilmiah Agrisains vol. 10 (2), hal. 78-82

12.   Aryantha,INP., S.Kusmaningati, A.B.Sutjiatmo, Y.Sumartini, A.Nursidah and S.Narvikasari, 2010, The effect of Laetiporus sp. (Bull. ex Fr.) Bond. Et Sing. (Polyporaceae) extract on total blood cholesterol level, Biotechnology, 9(3): 312-318

13.   Viera, B.V.E., Madayanti, F., Aryantha, I.N.P., & Akhmaloka, 2012, Succession of eukaryotic communities during traditional composting of domestic waste based on PCR-DGGE analysis, J. Pure Appl. Microbiol., 6(2), 525-536.

14.   Rashid, S.A.,Ibrahim D., & Aryantha, I.N.P., 2013, Effect Of Cultural Conditions On Lovastatin Production By Aspergillus Niger Sar I Using Combination Of Rice Bran And Brown Rice As Substrate, International Journal of Applied Biology and Pharmaceutical Technology, 4(2) : 150-156

SEMINAR 15.       Aryantha, IP., Rayati D.J, & P. Arbianto,  2001, The optimization of nutrition factors in spore production of Paecilomyces fumosoroseus (Wize) Brown-Smith with submerged-surface fermentation system, Proceedings of the Fifth symposium on Agri-Bioche, 2001, Tokyo, March 11, 2001. p. C11 16.       Aditiawati,P., D. Indriani & IP Aryantha, 2002, Growth optimization of biosurfactant producing bacteria for oil sludge bioremediation, International seminar on Environmental technology and management, January 9-10, 2002, Bandung, Indonesia 17.       Firman, AP & IP Aryantha, 2003, Eksplorasi dan isolasi ensim glukosa oksidase dari fungi imperfekti genus Penicillium dan Aspergillus indigenus, Prosiding Pertemuan Ilmiah Tahunan, PERMI, Bandung, Agust 29-30, 2003 18.       Aryantha, I.P. & S. Widayanti, 2004, Deteksi senyawa lovastatin pada kultur miselium dan tubuh buah jamur Tiram (Pleurotus ostreatus), PROCEEDING SEMINAR MIPA IV, Bandung, 6 – 7 Oktober 2004, Hal : 560-563 19.       Aryantha, IP., Y. Mulyani & R. Ariffudin, 2005, Aplikasi penanda molekuler mikrosatelit dan RAPD untuk standarisasi bibit jamur kuping (Auricularia sp), Makalah disajikan dalam Seminar hasil riset unggulan ITB tahun 2003-2004, Aula Barat ITB 24 Agustus 2005 20.       Aryantha, IP. & S.Kusmaningati, 2005, Efek pemberian ekstrak Ganoderma terhadap  kadar gula darah tikus putih Wistar, Poster Ilmiah disajikan dalam Kongres Pertemuan Ilmiah Tahunan, PERMI, Denpasar 25-27 Agustus 2005 21.       Aryantha, IP., S. Harjati, Y. Setiadi & N. Kristanti, 2005, Biodegradasi serat sawit dengan fungi lignoselulotik sebagai “bulking agent” media tanam tomat dan jagung, Poster Ilmiah disajikan dalam Kongres Pertemuan Ilmiah Tahunan, PERMI, Denpasar 25-27 Agustus 2005 22.       Aryantha, IP., 2005, Pengembangan produk kesehatan dari Shiitake, Prosiding Lokakarya Pengembangan Prpoduk dan Industri Jamur Pangan, BPPT Jakarta 1-2 Agustus 2005 23.       Aryantha, IP., 2005, Development of nutriceutical based on indonesian edible mushroom, Proceeding of China-Asean Workshop on Development of Edible Mushroom Industry, BPPT Jakarta 26-27 September 2005 24.       Cahyadi, Y &, IP. Aryantha, 2006, Biodegradation Of Cyanide By Bacterial Isolate From Tapioca Waste Origin From Tasikmalaya-West Java, International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java 25.       Kuswytasari ND, IP. Aryantha, & M. R. Moeis, 2006, The presence of bacterial endosymbiont in Aphid species from Lembang, West Java, International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java 26.       Nahampun, H.N., W. Widyasari, S. Suhandono & IP. Aryantha, 2006, Screening soft rot bacteria which utilize palatinose and cloning Pali (Sucrose ISomerase) gene from Erwinia rhapontici, International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java. P. 167 27.       Novilla, A., IP. Aryantha, & DI Astuti, 2006, The lactic acid bacteria as biocontrol agent against Salmonella typhi (Le Minor & Popoff), International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java 28.       Yuli, A.B. Sutjiatmo, IP. Aryantha, 2006, Effect of oyster mushroom mycelial water extract on total blood cholesterol level of Wistar female rats, International Conference on Mathematics and Natural Sciences, 29-30 Nov. 2006, Bandung West Java. P. 160. 29.       Aryantha, INP, S. Kusmaningati, A.B. Sutjiatmo & Y. Sumartini, 2007, The effect of Laetiporus sp. {Polyporeaceae} extract on total blood cholesterol level of Wistar female rats, Proceeding of IOCD International Symposium and Workshop Biology, Chemistry, Pharmacology and Clinical Study of Asian Plants, Seminar of Indonesian Medicinal Plants XXXI, Surabaya, Indonesia 9-11 April 2007. 30.       Aryantha, INP, M. Sidharta and S.K.Chaerun, 2007, Lovastatin production by Laetiiporus sp. {Bull. Ex. Fr.} Bond et Sing.in various solid substrates, Asian Mycological Congress (AMC), Penang Malaysia, 2-6 December 2007 31.       Aryantha, INP and D. Karamita, 2007, The effect of Gamma (γ) radiation on Lovastatin production by Laetiporus sp. {Bull. Ex. Fr.} Bond et Sing in sub,erged culture, Asian Mycological Congress (AMC), Penang Malaysia, 2-6 December 2007 32.       Aryantha, INP, T. Yuliana & D. Ismidianti, 2007, Pengaruh bakteri rhizozfir Bacillus sp. (ISolat 1.7) terhadap pertumbuhan vegetatif kedelai {Glycine max (L) Merill cv Willis, Kongres Nasional Himpunan Ilmu Tanah Indonesia IX, 5-7 Desember 2007, Yogyakarta 33.       Aryantha, INP, T. Yuliana & D. Ismidianti, 2007, Pengaruh inokulasi silang bakteri nodul dari berbagai tanaman leguminosae terhadap pertumbuhan tanaman kedelai hitam {Glycine sojae (Moench) FJ Herm, Kongres Nasional Himpunan Ilmu Tanah Indonesia IX, 5-7 Desember 2007, Yogyakarta 34.       Aryantha, INP., N.D.P. Pangesti, S.K. Chaerun, R.R. Esyanti, K.Toyota, 2008, Biogeochemical and agricultural significance of sediments : microbial diversity and activity of sediment from three largest dam reservoirs (Saguling , CIrata and Jatiluhur) in West Java Province, Indonesia, 2nd USM Penang Inernationl Post Graduate Convention, 18-20 June 2008 35.       Jumiarni, D., S.K. Chaerun, INP Aryantha, R.R. Esyanti, T. Sato, 2008, Sediment from three largest dam reservoirs (Saguling , CIrata and Jatiluhur) in West Java Province, Indonesia as natural biomineral fertilizers : an agricultural implication, 2nd USM Penang Inernationl Post Graduate Convention, 18-20 June 2008 36.       Chaerun, S.K., INP Aryantha, R.R. Esyanti, K. Toyota & T. Sato, 2008, Chemical and mineralogical characterization of sediment from three largest dam reservoirs (Saguling , CIrata and Jatiluhur) in West Java Province, Indonesia, 2nd USM Penang Inernationl Post Graduate Convention, 18-20 June 2008 37.       Chaerun, S.K,  INP Aryantha, R.R. Esyanti1, K. Toyota and T. Sato, 2008, Biogeochemistry of sediments II: mineralogical and chemical characterization of sediments from three largest dam reservoirs (Saguling, Cirata, Jatiluhur) in West Java Province, Indonesia, The 12th International Symposium on Microbial Ecology (ISME 12), Cairns, Australia, August 17 - 22, 2008 38.       Umrah, T. Anggraeni, R.R. Esyanti, INP Aryantha, 2008, The potential of Dolichoderus thoracicus Smith as a vector in the spread of Trichoderma spp. For controling Phytophthora palmivora (E.J. Butler) black pod doseases on cocoa, Proceedings of International Symposium on Tropical and Agroforests under global change, 5-9 October 2008, Kuta Bali Indonesia. 39.       Miryanti A. and I NP Aryantha; 2008, Proteolytic bacteria Study In Production Of Cheese By Fermentation; 2nd International Conference on Mathematics and Natural Sciences (ICMNS), Bandung 2008 40.       Supriatin,Y.  I NP Aryantha, Siti Khadijah Chaerun; 2008, Study Of Methane Production By Methanogenic Consortium Bacteria With A Substrate Of Jatropha Curcas Seed Pressed Cake; 2nd International Conference on Mathematics and Natural Sciences (ICMNS), Bandung, 2008 41.       Parnidi, Rizkita R. Esyanti, I NP Aryantha, Siti Khadijah Chaerun; 2008, Influence Of Sediment Application Collected From Saguling Dam Reservoir (West Java, Indonesia) As Natural Biomineral Fertilizer On Growth Of Rice (Oryza sativa) variety Situ Bagendit; 2nd International Conference on Mathematics and Natural Sciences (ICMNS), Bandung, 2008 42.       Sanjaya, RT.  Rizkita R. Esyanti, INP Aryantha, Siti Khadijah Chaerun, Parnidi, Deri Hendrawandi; 2008, Influence Of Sediment Application Amended With Azotobacter Chroococcum As Biomineral Fertilizers On Growth Responses And Productivity Of Red Chili Plant; 2nd International Conference on Mathematics and Natural Sciences (ICMNS), Bandung, 2008 43.       Oktari, A.  I NP Aryantha; 2008, The Study Of Sulphur Bacteria To Generate Electrical Energy In Microbial Fuel Cell (MFC) System; 2nd International Conference on Mathematics and Natural Sciences (ICMNS), Bandung, 2008 44.       Jamilah and INP Aryantha; 2008, The Study Of Hydrogen Bacteria On Producing Electrical Energy In Microbial Fuel Cell (MFC) System; 2nd International Conference on Mathematics and Natural Sciences (ICMNS), Bandung, 2008 45.       Aryantha INP and Jamilah; 2008, The Study Of Microbial Fuel Cell (MFC) Using Microbial Consortium From Organic Wastes; 2nd International Conference on Mathematics and Natural Sciences (ICMNS), Bandung, 2008 46.       Pangesti, NPD,  Tri Yuliana, Firman Ardiansyah, Rizka Utami Putri, Rizkita Rachmi Esyanti, INP. Aryantha; 2009, The Potency of Endophytic Bacteria from Palm Oil (Elaeis guineensis Jacq.) to Inhibit the Growth of Ganoderma boninense, to Fix Nitrogen and to Produce Phytohormone In Vitro; International Conference And Exhibition-Science & Technology In Biomass Production: Optimizing University-Industry Collaboration, ITB Bandung, 25-26 November 2009 47.       Aryantha INP and Reny Maria Angraeni; 2009, Electrical Potential by Ammonifying Bacteria (Xanthomonas sp) in Microbial Fuel Cell (MFC) System; International Conference And Exhibition-Science & Technology In Biomass Production: Optimizing University-Industry Collaboration, ITB Bandung, 25-26 November 2009 48.       Puspitasari I.N and INP Aryantha; 2009, Developing Microbial Fuel Cell (MFC) System Using Gamma Irradiated Nitrogen Bacteria (Xanthomonas sp.); International Conference And Exhibition-Science & Technology In Biomass Production: Optimizing University-Industry Collaboration, ITB Bandung, 25-26 November 2009 49.       Munfarida I and INP. Aryantha; 2009, Electricity Generation from Iron-Bacteria Fuel Cell System; International Conference And Exhibition-Science & Technology In Biomass Production: Optimizing University-Industry Collaboration, ITB Bandung, 25-26 November 2009 50.       Swandjaja, L.W.P.,  Rizkita Rachmi Esyanti, Parnidi, Siti Khodijah Chaerun and INP. Aryantha; 2009, The Role of Bacillus cereus in Increasing Paddy Rice Growth and Productivity; International Conference And Exhibition-Science & Technology In Biomass Production: Optimizing University-Industry Collaboration, ITB Bandung, 25-26 November 2009 51.       Karlesa A. and INP Aryantha; 2009, The Growth of Lyophyllum sp. on Several Solid Substrates; International Conference And Exhibition-Science & Technology In Biomass Production: Optimizing University-Industry Collaboration, ITB Bandung, 25-26 November 2009 52.       Asarina S. and INP Aryantha; 2009, The Electrical Voltage Production of Gamma-Irradiated Iron Bacteria (Pseudomonas sp) in Microbial Fuel Cell (MFC) System; International Conference And Exhibition-Science & Technology In Biomass Production: Optimizing University-Industry Collaboration, ITB Bandung, 25-26 November 2009 53.       Annisa J. and INP Aryantha; 2009, Optimization of Microbial Fuel Cell (MFC) Using Gamma-Irradiated Hydrogen Bacteria (Pseudomonas hydrogenovora); International Conference And Exhibition-Science & Technology In Biomass Production: Optimizing University-Industry Collaboration, ITB Bandung, 25-26 November 2009 54.       Aryantha INP and Jamilah; 2009, Modification of Membrane, Cathode Electrolite and Anodic Materials in Microbial Fuel Cell (MFC) System; International Conference And Exhibition-Science & Technology In Biomass Production: Optimizing University-Industry Collaboration, ITB Bandung, 25-26 November 2009 55.       Melia,S and INP. Aryantha, 2010, The Effects of Chitosan on Antifungal Activity of Trichoderma harzianum Rifai against Fusarium oxysporum, The 2nd International Biotechnology & Biodiversity Conference (BIOJOHOR 2010), July 6-8, 2010, Johor Bahru, Johor, Malaysia 56.       Aryantha, INP, R. Canto and B. Anggoro, 2010, The Effect of Magnetic Field Induction on Fruiting Body Emergence and Lovastatin Production by Laetiporus sp., 4th International Conference on Mathematics and Natural Sciences (ICMNS), Bandung, 23-25 November 2010 57.       Aryantha, INP and R. D. Nurhapsari, 2010, The optimization of mycelial pellet biomass production on Penicillium roqueforti (Thom) and Rhizopus oligosporus (Saito), 4th International Conference on Mathematics and Natural Sciences (ICMNS), Bandung, 23-25 November 2010 58.       Aryantha, INP., Siti Khodijah Chaerun, Rizkita Rachmi Esyanti, Rizka Utami Putri, Myrna Haryathi, 2010, The Potency of Sediment from Saguling Dam (West Java) as Organic Fertilizer for Rice Plant, 4th International Conference on Mathematics and Natural Sciences (ICMNS), Bandung, 23-25 November 2010 59.       Kurniawan, C., IN.P. Aryantha dan S. Asarina, 2010, Kajian potensi limbah whey sebagai sumber tenaga listrik dalam sistim mfc (microbial fuel cell) bakteri asam laktat, 2nd International Conference on Bioscience and Biotechnology, Denpasar 23-24 September 2010 60.       Aryantha INP and S. Asarina, 2010, DEVELOPMENT OF MICROBIAL FUEL CELL WITH BACTERIAL CATHODIC, The 4th International Conference on Engineering and Management, Senggigi, Lombok, 1-4 December 2010. 61.     Aryantha, INP and Y. Maryana, 2012, Optimasi Produksi Tubuh Buah Jamur Tiram Putih (Pleurotus ostreatus), Prosiding Seminar Nasional Mikologi, 15-16 Mei 2012, Universitas Jenderal Seodirman, Purwokerto. 62.     Aryantha, INP, 2012, Cendawan sumber pangan masa depan : bergizi dan menyehatkan, Invited speaker, Seminar Nasional Mikologi, 15-16 Mei 2012, Universitas Jenderal Seodirman, Purwokerto. IPR 1.        Nganro, .N R., Aryantha, INP Sukrasno, 1998, Teknologi mikroba probiotik indigenous untuk aplikasi pertanian, perikanan, dan bioremediasi (Trade Secret dan Lisensi teknologi : Kontrak No. 004/KMHaKI-ITB/PKP/II/00) 2.        Aryantha , INP., D. P. Lestari, M. Gantina, 2001 , Biofungicide from indigenous microbes for controlling root rot diseases ( Patent :Filing Date Ditjen HAKI : No. File Date P20010211) 3.        Aryantha , INP., 2003 , Non-chemical extraction method for extracting bioactive compound of medicinal mushroom (Patent : Filing Date Ditjen HAKI No. File Date P00200300565) 4.        Suhermiyati, S, Aryantha, INP., E. Musnandar, Soeharsono, U.D.Rusdi, S. Darana  2008, Poultry feedstock component from bioprocessed cocoa husk waste (Patent ID : 0021180) 5.        Aryantha, INP, Jamilah, I. Munfarida, R.M.Angraeni & A. Oktari,  2009, Sel bahan bakar menggunakan bakteri litotrof (Patent : No. File Date P00200900) 6.        Aryantha, INP & Waluyajati, R, 2010, Proses Produksi Senyawa Antikolesterol Lovastatin Dari Kultur Biomasa Jamur Pleurotus ostreatus (No. File Date: P00201000788) 7.        Aryantha, INP., D.P. Lestari & N. Puri, 2011, Phytotohormone producing bacterial package from indigenous isolates (Patent ID : P0027424 B) BOOK (BOOK CHAPTER) 1.         Prosiding Seminar Tantangan Dan Prospek Hayati Dalam Meningkatkan Ketahanan Ekonomi Nasional, 30 Juni & 1 Juli 1998 by Institut Teknologi Bandung, (Editor : Sukrasno, I Nyoman Aryantha, Noorsalam R. Nganro) ISBN 9798591836 (979-8591-83-6) Hardcover, Diterbitkan atas kerjasama LP-ITB, LPM-ITB, [dan] PPAU Ilmu Hayati LP-ITB 2.         Teknologi budidaya jamur, 2000, KPP Ilmu Hayati ITB, Bandung 3.         Dasar-dasar pembibitan jamur, 2000, KPP Ilmu Hayati ITB, Bandung 4.         Protein sel tunggal berbasis biomasa miselium, 2006, Program Hibah Kompetensi DIKTI 5.         Inovasi Pengelolaan Mikroba (Chapter dalam buku EKONOMI INOVASI), 2010, Lembaga Pengembangan Inovasi dan Kewirausahaan ITB AWARD 1.       Penghargaan atas hasil lisensi karya Kekayaan intelektual ITB 2001 2.       Adikhara Rekayasa Award Persatuan Insinyur Indonesia (PII) , 2003 3.       AFEO Award Asean Federation of Engineering Organization 2003 4.       Satya Lencana Pemerintah RI 2004 5.       Dosen Berprestasi ITB Tahun 2012 Bidang Pengabdian kepada Masyarakat Kategori Penerapan Teknologi Bandung, April 2013 I Nyoman P. Aryantha